Review



alt-r crispr custom guide rnas  (Integrated DNA Technologies)


Bioz Verified Symbol Integrated DNA Technologies is a verified supplier
Bioz Manufacturer Symbol Integrated DNA Technologies manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Integrated DNA Technologies alt-r crispr custom guide rnas
    Alt R Crispr Custom Guide Rnas, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/Alt-R+CRISPR+Custom+Guide+RNAs/custom%40alt-r-crispr-custom-guide-rnas%4010%2E64898%2F2026%2E08%2E10%2E743836
    Average 92 stars, based on 34 article reviews
    alt-r crispr custom guide rnas - by Bioz Stars, 2026-11
    92/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs
    Article Snippet: Nucleofection was performed by pooling 1 × 10 5 HDFa cells per condition and spun down at 300 g for 10 min, washed with 1× PBS, spun again, then resuspended in P2 primary cell solution (10 μl per condition, Lonza). .. DNA mixtures were prepared by combining 50 pmol of chemically synthesized guide RNA (IDT or Synthego, Supplementary Table ) with 1 μg of in vitro transcribed base editor mRNA and P2 primary cell solution into a total volume of 12 μl. ..

    In Vitro:

    Article Title: High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs
    Article Snippet: Nucleofection was performed by pooling 1 × 10 5 HDFa cells per condition and spun down at 300 g for 10 min, washed with 1× PBS, spun again, then resuspended in P2 primary cell solution (10 μl per condition, Lonza). .. DNA mixtures were prepared by combining 50 pmol of chemically synthesized guide RNA (IDT or Synthego, Supplementary Table ) with 1 μg of in vitro transcribed base editor mRNA and P2 primary cell solution into a total volume of 12 μl. ..

    Modification:

    Article Title: Long-read sequencing across the C9orf72 ‘GGGGCC’ repeat expansion: implications for clinical use and genetic discovery efforts in human disease
    Article Snippet: .. Oligos comprising the guide RNA (crRNA and tracrRNA) were obtained from Integrated DNA Technologies containing an Alt-R modification to prevent RNase degradation. .. Cas9 Nuclease, S. pyogenes, was obtained from New England Biolabs (PN M0386S).

    Incubation:

    Article Title: ADAP1 promotes latent HIV-1 reactivation by selectively tuning a T cell signaling-transcriptional axis
    Article Snippet: .. TracrRNA and guide RNA (IDT) were mixed and heated at 95°C for 5 min. Cas9 (IDT) was added to the RNA mixture and incubated at room temperature for 20 min. CD4 + T cells were washed with PBS and resuspended in Buffer T of Neon Transfection System 10 mL kit (ThermoFisher). .. Pre-assembled Cas9-gRNA protein-RNA complexes were electroporated into cells using Neon equipment (ThermoFisher).

    Article Title: ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS–ERK–AP-1 T cell signaling-transcriptional axis
    Article Snippet: .. TracrRNA and guide RNA (IDT) were mixed and heated at 95 °C for 5 min. Cas9 (IDT) was added to the RNA mixture and incubated at room temperature for 20 min. CD4 + T cells were washed with PBS and resuspended in Buffer T of Neon Transfection System 10 ml kit (Thermo Fisher). .. Pre-assembled Cas9-gRNA protein-RNA complexes were electroporated into cells using Neon equipment (Thermo Fisher).

    Transfection:

    Article Title: ADAP1 promotes latent HIV-1 reactivation by selectively tuning a T cell signaling-transcriptional axis
    Article Snippet: .. TracrRNA and guide RNA (IDT) were mixed and heated at 95°C for 5 min. Cas9 (IDT) was added to the RNA mixture and incubated at room temperature for 20 min. CD4 + T cells were washed with PBS and resuspended in Buffer T of Neon Transfection System 10 mL kit (ThermoFisher). .. Pre-assembled Cas9-gRNA protein-RNA complexes were electroporated into cells using Neon equipment (ThermoFisher).

    Article Title: ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS–ERK–AP-1 T cell signaling-transcriptional axis
    Article Snippet: .. TracrRNA and guide RNA (IDT) were mixed and heated at 95 °C for 5 min. Cas9 (IDT) was added to the RNA mixture and incubated at room temperature for 20 min. CD4 + T cells were washed with PBS and resuspended in Buffer T of Neon Transfection System 10 ml kit (Thermo Fisher). .. Pre-assembled Cas9-gRNA protein-RNA complexes were electroporated into cells using Neon equipment (Thermo Fisher).



    Similar Products

    alt-r crispr custom guide rnas  (Integrated DNA Technologies)
    92
    Integrated DNA Technologies alt-r crispr custom guide rnas
    Alt R Crispr Custom Guide Rnas, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/Alt-R+CRISPR+Custom+Guide+RNAs/custom%40alt-r-crispr-custom-guide-rnas%4010%2E64898%2F2026%2E08%2E10%2E743836
    Average 92 stars, based on 1 article reviews
    alt-r crispr custom guide rnas - by Bioz Stars, 2026-11
    92/100 stars
    		  Buy from Supplier
    guide rna grna sequences targeting hspb1  (Addgene inc)
    94
    Addgene inc guide rna grna sequences targeting hspb1
    Guide Rna Grna Sequences Targeting Hspb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/LSB-hsa-miR-299-5p+(Plasmid+%23103390)/pm42882493-70-0-19
    Average 94 stars, based on 1 article reviews
    guide rna grna sequences targeting hspb1 - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    plenticrisprv2 plasmid encoding guide rna targeting cypa  (Addgene inc)
    98
    Addgene inc plenticrisprv2 plasmid encoding guide rna targeting cypa
    Plenticrisprv2 Plasmid Encoding Guide Rna Targeting Cypa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/psPAX2+(Plasmid+%2312260)/bio_rxiv__64898__2026__10__07__757322-55-4-19
    Average 98 stars, based on 1 article reviews
    plenticrisprv2 plasmid encoding guide rna targeting cypa - by Bioz Stars, 2026-11
    98/100 stars
    		  Buy from Supplier
    small guide rnas sgrnas  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology small guide rnas sgrnas
    (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing <t>RNAs;</t> ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .
    Small Guide Rnas Sgrnas, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/Nanos3+CRISPR%2FCas9+KO+Plasmid/pmc13641165-565-6-10
    Average 93 stars, based on 1 article reviews
    small guide rnas sgrnas - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    guide rnas targeting ire1α  (Addgene inc)
    94
    Addgene inc guide rnas targeting ire1α
    (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing <t>RNAs;</t> ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .
    Guide Rnas Targeting Ire1α, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/ERN1+gRNA+(BRDN0001146341)+(Plasmid+%2376408)/pm42778064-90-1-10
    Average 94 stars, based on 1 article reviews
    guide rnas targeting ire1α - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    toronto knockout version 3 0 tkov3 guide rna guide rna grna 106 library  (Addgene inc)
    94
    Addgene inc toronto knockout version 3 0 tkov3 guide rna guide rna grna 106 library
    (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing <t>RNAs;</t> ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .
    Toronto Knockout Version 3 0 Tkov3 Guide Rna Guide Rna Grna 106 Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/Toronto+KnockOut+(TKO)+CRISPR+Library+-+Version+3+(Pooled+Libraries+%2390294%2C+%23125517)/10__1158_slash_2767___9764__crc___26___0150-55-12-22
    Average 94 stars, based on 1 article reviews
    toronto knockout version 3 0 tkov3 guide rna guide rna grna 106 library - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    guide rna product  (Qiagen)
    99
    Qiagen guide rna product
    (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing <t>RNAs;</t> ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .
    Guide Rna Product, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/RNeasy+MinElute+Cleanup+Kit/10__1186_slash_s13578___026___01632___w-237-8-18
    Average 99 stars, based on 1 article reviews
    guide rna product - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    crispr guide rna design tool  (Benchling Inc)
    86
    Benchling Inc crispr guide rna design tool
    (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing <t>RNAs;</t> ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .
    Crispr Guide Rna Design Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/crispr+design+tool/pmc13155868-62-34-40
    Average 86 stars, based on 1 article reviews
    crispr guide rna design tool - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    sirpα single guide rna  (Synthego Inc)
    86
    Synthego Inc sirpα single guide rna
    (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing <t>RNAs;</t> ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .
    Sirpα Single Guide Rna, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna/guide+rna+single+sirp%CE%B1/pm42320470-695-16-19
    Average 86 stars, based on 1 article reviews
    sirpα single guide rna - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing RNAs; ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .

    Journal: Molecular cell

    Article Title: DND1–NANOS3 shapes the PGC transcriptome via a heptanucleotide sequence in mRNA 3′-UTRs

    doi: 10.1016/j.molcel.2026.09.010

    Figure Lengend Snippet: (A) DND1 (purple) and NANOS3 (pink) residues interacting with the N3-DRE. Filled shading, residues substituted with alanine for functional analysis. (B) DND1–NANOS3 co-expression constructs with indicated mutations (top) or truncations (bottom). (C) Schematic of assays performed with cells expressing constructs from (B). (D) Assay results for DND1–NANOS3 constructs with DND1 point mutants: ( 1 ) IncuCyte growth assays; ( 2 ) expression of N3-DRE-containing RNAs; ( 3 ) protein levels of N3-DRE-containing targets (CDK1, EHMT2, and CNOT6; immunoblots in Fig. S5 ). (E) As in (D), with NANOS3 point mutations. (F) DND1 K196A loses selectivity for the N3-DRE G3 nucleotide in vitro . Error bars, K d confidence interval from MST data fitting. (G) As in (D), with DND1 or NANOS3 truncations. (H) Effects of DND1/NANOS3 mutations or deletions on RNA turnover. Left, median fold changes in k deg for each N3-DRE class, normalized to the corresponding No motif group. Middle, median fold changes in k syn . Right, median fold changes in transcript half-life. Corresponding violin plots and CDFs are shown in Figures S5F and S5G . (I) Summary of the functional impact of RNA-binding residue mutations and IDR deletion. Statistics (D-F): Cell growth: Scale bar, 400 μm (bottom right image only). CDFs: n, number of genes; ML, median log 2 -fold change; δ, Cliff’s delta; p , two-sided Wilcoxon rank-sum test. Protein quantification: Error bars indicate S.D. Two-sided Student’s t -test ( n = 3 independent experiments). Exact p -values are provided in Source Data. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S5 and Table S1 .

    Article Snippet: We purchased a cocktail of three small guide RNAs (sgRNAs) (Santa Cruz Biotechnology, Inc., sc-434074), cloned individual sgRNA plasmids, and used pairs of the sgRNAs for the targeted deletions of the ZF region of the Nanos3 locus.

    Techniques: RNA Binding Assay, Functional Assay, Analysis, Expressing, Construct, Western Blot, In Vitro, Residue